CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Relationship among neutrophil counts, autoantibody levels and disease progression in synovial fluids from patients with rheumatoid arthritis.
Autor/es:
GORLINO, C; DAVE M; TAMASHIRO, H; BLAS, R; MUNARRIZ A; PISTORESI, MC; DI GENARO, S
Lugar:
Mar del Plata
Reunión:
Congreso; LIX Reunión Científica de Inmunología.; 2014
Institución organizadora:
Sociedad Argentina de Inmunología.
Resumen:
Rheumatoid arthritis (RA), one of the most frequent autoimmune
disease, is a chronic inflammatory rheumatism hallmarked by
progressive and irreversible joint destruction. Among the numerous
autoantibodies associated with RA, anti-cyclic citrullinated peptide
antibodies (ACPA) are now recognized as the most diseasespecific.
The aim of this study was to determine the relationship
between neutrophil infiltration into inflamed joints and the presence
of ACPA in RA patients. Synovial fluid (SF) samples were obtained
from 42 patients (Male/Female: 8/34; mean age: 53±13 years) who
full-filled the American College of Rheumatology/European League
Against Rheumatism (ACR/EULAR) RA classification criteria. All
patients gave informed consent and the protocol of the study was
approved by the ethic board of IBYME (CE 003-2/2013). Disease
activity was evaluated by 28-joint count Disease Activity Score
(DAS-28) and erythrocyte sedimentation rate (ESR) was determined
by Westergren method. Immunoglobulin G (IgG) ACPA were
measured using QuantaLite CCP3 ELISA kit and total IgG levels
were determined by radial immunodiffusion assay. We found that
neutrophil numbers infiltrating inflamed joints correlated positively
with DAS-28, suggesting that the presence of high numbers of
these cells is related with severe disease manifestations (p