CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
A nanostructure from ascorbyl palmitate based-adjuvant boosts adaptive immunity by inducing an inflammatory effect at the injection site, both properties require the adaptor protein Myd88.
Autor/es:
SANCHEZ-VALLECILLO,MF; ULLO, G; PALMA, S; GONZÁLEZ-CINTADO,L; CHIODETTI, A; AGUIRRE,MV; MORÓN, G; ARDAVIN,C; PISTORESI, MC; MALETTO, B
Lugar:
Mar del Plata
Reunión:
Congreso; LXII Reunión de la Sociedad Argentina de Inmunología.; 2014
Institución organizadora:
Sociedad Argentina de Inmunología.
Resumen:
The approved adjuvants for human use induce weak cellular immune responses in subunit vaccines. Thus, the development of new adjuvant strategies is critical. Recently, we have showed that a nanostructure from 6-O-ascorbyl palmitate (Coa-ASC16), used as a platform to formulate CpG-ODN, improved notably its adjuvanticity; one of the possible mechanisms involved is the controlled release given by Coa-ASC16 formulation. In addition, Coa-ASC16 elicits local inflammatory response but how it is sensed is little understood. Here, we begin to understand the underlying mechanisms on the interaction between Coa-ASC16 and immune system after i.p injection. We previously found that the inflammatory response elicited by Coa-ASC16 (neutrophils and monocytes recruitment and IL-1β, IL-6, IL-12) was dependent on MyD88 adaptor protein. TLR2, TLR4 and TLR7 were dispensable for this inflammatory effect. The administration of Coa-ASC16 was associated with the local release of double-stranded DNA (Coa- ASC16 vs control 2.5±0.5 vs 0.5±0.1 (μg/mL) (p