Induction of Th2-mediated allergic asthma in a mouse strain highly susceptible to develop th1 responses

GODOY, GJ; SANCHEZ, L; GARCIA, L; MALDONADO, C; MOTRICH, RD; RIVERO, VE

Mar del Plata

Congreso; LIX Reunión Científica Anual de la SAIC ? LXII Reunión Científica Anual de la SAI; 2014

Sociedad Argentina de Investigación Clínica y Sociedad Argentina de Inmunologia

NOD mice are highly susceptible to chronic inflammation disorders mostly mediated by Th1 responses. This increased susceptibility has been thought to be related to defects in regulatory T cells (Tregs). Recent findings evidenced that Tregs subsets are heterogeneous and that different Tregs subsets able to specifically regulate Th1, Th2 and Th17 immune responses would exist.In this work, we developed a model of allergic asthma following established immunization protocols with OVA plus aluminum salts in NOD, C57BL/6 and Balb/c mice. We aimed to analyze if regulatory defects observed in NOD mice were general or just restricted to Th1 responses. After immunization, mice from every strain showed clinical signs and symptoms of allergy when challenged with OVA intranasally. Although increased leukocyte counts in broncoalveolar lavage (BAL) were detected in both immunized NOD and Balb/c mice, the highest levels were seen in Balb/c mice (p