CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Cosntitutive Beta Defensin 1 is modulated by Candida albicans in Vulvovaginal Candidiasis.
Autor/es:
MIRÓ MS; ICELY PA; VIGEZZI C; RODRIGUEZ EMILSE; SOTOMAYOR CE
Lugar:
Mar del Plata
Reunión:
Congreso; LIX Reunión Anual de la Sociedad Argentina de Investigación Clínica y LXII reunión científica anual de la Sociedad Argentina de Inmunología,; 2014
Institución organizadora:
Sociedad Argentina de Investigación Clínica y Sociedad Argentina de Inmunología,
Resumen:
β-defensins (BDs) are antimicrobial peptides expressed by epithelial barriers and immune cells that play an important role in the innate host defense. BDs exert antimicrobial and chemoattractant activities. Human BD1 (mouse BD1 orthologous), unlike other BDs which are induced by microbial compounds or inflammatory stimuli, is constitutively expressed with constant levels in normal tissue. Candida albicans (Ca) is a common commensal of mucosal surfaces and the most frequent cause of Vulvovaginal Candidiasis (VVC). The role of BD1 during this pathology is unclear and modulation of BD1 by fungal factors remains to be elucidated. We aimed to study Ca ability to modulate the expression of BD1 In Vivo in a mouse model of VVC and In Vitro using a cervicovaginal human epithelial cell line. Female C57BL/6 mice were used. Estrus phase was induced by estradiol injections. Infected animals (Inf) were intravaginally inoculated with 5.106 Ca ATCC 36801 at day (D) 0. Treated uninfected (Treat) mice were used as controls. At D 2, 4 and 8 post infection (pi), vaginal lavage was obtained to evaluate fungal burden (CFU) and intracellular expression of mBD1 (flow cytometry) in Cytokeratin+ cells (epithelial cells) and Gr1+ (myeloid cells). BD1 was increased in epithelial and Gr-1+ cells in Inf mice at D2 pi (p