PD-1+TIM-3+ T cells from 4T1 tumor-bearing mice display features of exhaustion and increased expression of immunomodulatory molecules.

CANALE F; RAMELLO, MARIA CECILIA; GOROSITO SERRAN, MELISA; ARAUJO CINTIA; TOSELLO BOARI, JIMENA; ACOSTA RODRIGUEZ E V; GRUPPI ADRIANA; MONTES CAROLINA L

Mar del Plata

Jornada; LXII Reunión Anual de la Sociedad Argentina de Inmunología (Mar del Plata, Noviembre, 2014); 2014

Sociedad Argentina de Inmunologia

PD-1+TIM-3+ T cells from 4T1 tumor-bearing mice display features of exhaustion and increased expression of immunomodulatory molecules. Canale, Fernando; Ramello, M Cecilia; Gorosito Serran, Melisa; Araujo Furlan, Cintia; Tosello, Jimena; Acosta Rodriguez, Eva; Gruppi, Adriana; Montes, Carolina. CIBICI-CONICET. Departamento de Bioquímica Clínica. Facultad de Ciencias Químicas. Universidad Nacional de Córdoba. T cell exhaustion is a T cell dysfunction state that arises during chronic infections and cancer. It is characterized by poor effector functions and sustained expression of inhibitory receptors (iRs) like PD-1 and TIM-3. Based on our previous findings, we hypothesize that these apparently dysfunctional T cells may have modulatory functions. In this work we studied phenotype and functional characteristics of tumor-infiltrating lymphocytes (TILs) and T cells from tumor-draining lymph nodes (dLN) of 4T1 mammary carcinoma bearing mice; comparing PD-1+TIM-3+ double positive (DP) cells with PD-1-TIM-3- double negative (DN) cells. Within DP TILs population we found higher % of cells expressing iRs like 2B4, LAG-3 and KLRG-1 compared to DN T cells, being 2B4 and LAG-3 more frequent in CD8+ DP T cells (p