CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
IL-17 A and F sustain CD8+ T cell response to T cruzi by modulating survival, differentiation and exhaustion partially Through CD8+ T cell intrinsic IL-17RA signaling
Autor/es:
TOSELLO BOARI J; ARAUJO FURLÁN CINTIA;; FIOCCA VEMENGO F; RAMELLO MC; GOROSITO SERRAN M; MONTES CL; GRUPPI A; ACOSTA RODRIGUEZ EV
Reunión:
Congreso; LIX Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica. LXII Reunión Anual de la Sociedad Argentina de Inmunologia; 2014
Resumen:
The IL17 family contributes to host defense against intracellular
microbes by inducing inflammation and immune cell
recruitment. We described a novel mechanism by which IL17RA
is critically involved in the induction of CD8+ T cell (CTL) responses.
We demonstrated that compared to WT mice, IL17RA
KO (KO) mice infected with T. cruzi (Tc) developed a reduced
frequency of Tc-specific CTL. Here, we investigated whether the
IL17RA requirement for robust CTL responses is CTL intrinsic
and which particular IL17RA-signaling cytokine is involved. We
determined that IL17RA is constitutively present in CTL and
induced upon Tc infection. Next, we performed adoptive transfer
assays to determine if IL17RA expression on the CTL itself
provided an advantage to survive and respond to Tc. First, we
transferred CTL from CD45.1 WT mice into CD45.2 WT and KO
host and analyzed upon infection, the contribution of the injected
cells to the CTL response. After 20 days post infection (dpi), the
contribution of injected CD45.1 CTL to Tc-specific CTL pools was
higher in KO than in WT hosts (blood p=0.003; spleen p=0.011).
Convincingly, we transferred equal numbers of CTL from CD45.1
WT and CD45.2 KO mice into CD45.1/2 hosts. After 20dpi,
injected KO CTL were significantly outcompeted by their WT
counterparts (blood p=0.016; spleen p=0.014). To elucidate the
IL17 cytokine critical for CTL responses, we evaluated how lack
of IL17A/F affected the course of Tc infection. Infected IL17A/F
KO mice showed the same phenotype than infected KO mice
(i.e. increased tissue parasitism, reduced total and Tc-specific
CTL and exhausted CTL phenotype). Finally, we determined that
purified naive CTL upregulated IL17RA expression upon TCR
engagement and become responsive to rIL17A that diminished
the% of AnnexinV+ cells (p