A viral dsRNA analog inhibits tumor growth and increases tumor-bearing mice survival

DAVID ANDRÉS NOCERA; NICOLÁS G NÚÑEZ; GERARDO GATTI; ESTEFANÍA ZACCA; GABRIEL MORÓN; MARIANA MACCIONI

Puerto Varas

Congreso; CHILEAN SOCIETY FOR CELL BIOLOGY XXVII ANNUAL MEETING October, 23 - 27th, 2013 Puerto Varas; 2013

CHILEAN SOCIETY FOR CELL BIOLOGY

Introduction: The use of viral dsRNA mimetics in cancer immunotherapy has long been explored empirically. The goal of this work was to analyze the effect of administered Polyadenylicpolyuridylic acid (pAU), a TLR3 ligand, on the specific immune response against tumor antigens. Material and Methods: B16 murine melanoma cells expressing Ovalbumin (OVA) were inoculated s.c. in wild-type, genetically modified and athymic mice. When tumors reached a measurable size (~day 10 p.i), a group of mice from each strain was intratumorally treated with 50 μg of pAU (n=10, 4 doses every two days). To study the specific immune response against OVA, in vivo proliferation assays were performed using CFSE-labeled and adoptively transfered T cells. Results: pAU-treated wild-type mice showed a significantly reduced tumor growth as well as a longer survival compared to controls (p