Chlorpyrifos pesticide effect on villous trophoblast and explant cultures from human normal term placentas

MAGALI E RIDANO; ANA C RACCA; RICARDO FRETES; ESTELA BEVILACQUA; SUSANA GENTI-RAIMONDI; GRACIELA M PANZETTA-DUTARI

Foz de Iguazú

Congreso; V SLIMP - IV LASRI Meeting 2013; 2013

Latin American Society for Maternal Fetal Interaction and Placenta

Epidemiological data suggest that chronic exposure to Chlorpyrifos (CPF), one of the most used pesticides, leads to increased risk of pregnancy disorders. Here, we assessed the effects of CPF on villous cytotrophoblasts (CTBs) and explants from human normal-term placentas. This study was approved by the Hospital Privado Ethics Committee, Argentina. Morphology, cell viability and gene expression were analyzed in samples exposed to increasing CPF concentrations up to 100μM for up to 64h. In these conditions, CTBs differentiated to syncytium-like structures as the non-exposed control; showed higher expression of P-gp (ATP-binding cassette sub-family B member 1), ABCG2 (ATP binding cassette sub-family G member 2) and βhCG (human chorionic gonadotropin ß-subunit); and did not present signs of cellular dead. However, morphological alterations and cellular dead signs were observed in placental villous explants exposed to the same CPF concentrations. These results support and extend our previous findings obtained in JEG-3 cells and suggest that CPF cytotoxicity on trophoblast may be influenced by surrounding components of the placental villi. Supported by CONICET, FONCyT, MinCyT & SECyT-UNC.