Chlorpyrifos triggers anti-oxidative defense mechanisms in JEG-3 cells

CHIAPELLA G; FLORES-MARTÍN J; RIDANO M; REYNA L; PANZETTA-DUTARI G; MAGNARELLI G; GENTI RAIMONDI S

Foz de Iguazú

Congreso; V SLIMP - IV LASRI Meeting 2013; 2013

Latin American Society for Maternal Fetal Interaction and Placenta

A number of physiological and pathological processes produce reactive oxygen species which can promote multiple forms of oxidative injury. Here, we analyzed the effect of chlorpyrifos (CPF) on the JEG-3 cell antioxidant defenses. Thus, in addition to acetylcholinesterase (AChE) activity, reduced glutathione (GSH) content and the activities of antioxidant enzymes such as catalase (CAT) and carboxylsterase (CE) were measured. Furthermore, hemo-oxygenase 1 (HO-1), and nuclear factor E2-related factor 2 (Nrf2) transcript levels were analyzed. Also, Nrf2 western blot was performed. The results showed that CPF inhibited AChE activity with no changes in CE activity. An alteration in CAT activity and GSH concentration were found. In addition, CPF increased HO-1 mRNA and Nrf2 at both mRNA and protein levels. The effect on CAT upregulation was attenuated in N-acetylcysteine-treated JEG-3 cells. These studies establish a molecular mechanism for the regulation of CPF detoxification in JEG-3 cells and have implications for controlling the oxidative stress tolerance of xenobiotics in trophoblast cell.