Differential regulation of neuronal and microglial cell death by Toll-like receptors (TLRs)

BUSSI C., PERALTA RAMOS J., GAVIGLIO EA., ARROYO DS., RODRIGUEZ-GALÁN MC., IRIBARREN P.

Congreso; LXI Reunión Anual de la Sociedad Argentina de Inmunología; 2013

Sociedad Argentina de Inmunología

Introduction: TLRs are a family of innate immune system receptors that respond to pathogen and damage-associated molecular patterns. TLR signaling plays an elemental role in innate and adaptative immune responses. TLRs are expressed in the central nervous system (CNS) by neurons, microglia, astrocytes, and cerebral vascular cells. Increasing evidences support the idea that TLRs are pivotal in brain pathologies such as neurodegenerative disorders. Purpose: The aim of this study was to evaluate the participation of TLRs in the regulation of neuronal and microglial cell death. Experimental procedure: Neuronal and microglial cells were stimulated with PGN (TLR2 agonist) and LPS (TLR4 agonist) at different times and doses. Cell death was evaluated using Annexin V/propidium iodide staining and subsequent flow cytometric analysis. Results: Our preliminary results show that LPS stimulation for 24 and 48 h induces a significant increase (p