B cell deficient mice infected with Trypanosoma cruzi develop a proinflammatory T cell response potentially harmful for the host

GOROSITO SERRÁN, MELISA; TOSELLO BOARI, JIMENA; BERMEJO, DANIELA ANDREA; RAMELLO, MARÍA CECILIA; FIOCCA VERNENGO, FACUNDO; AMEZCUA VESELY, MARÍA CAROLINA; BECCARIA, CRISTIAN GABRIEL; MONTES, CAROLINA LUCÍA; ACOSTA RODRÍGUEZ, EVA VIRGINIA; GRUPPI, ADRIANA

Los Cocos

Congreso; LXI REUNIÓN CIENTÍFICA ANUAL DE LA SOCIEDAD ARGENTINA DE INMINOLOGÍA ? SAI; 2013

Sociedad Argentina de Inmunología

B cell-deficient mice (C129.BL6mMT) infected with T. cruzi, have more parasitemia than C57BL/6 wild type (WT) mice and accelerated mortality. To assess possible causes of this increased susceptibility, we analyzed the production of cytokines and the CD4+T-cell response in infected mMT and WT mice. Mice were infected by i.p. injection of 10000 trypomastigotes Y-br strain. Groups of 4-7 mice were sacrificed on days 4, 9, 15, 20 post-infection (pi); splenocytes were obtained and CD4+ T cells were studied by flow-cytometry. In addition, serum samples were obtained to quantify IFNg and TNF by ELISA. Both infected mice strains presented by day 9 (pi) increased amounts of serum IFNg, being higher in mMT mice (p