CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
PEPTIDOGLYCAN FROM STAPHYLOCOCCUS AUREUS INDUCES RECRUITMENT OF LEUKOCYTES TO THE CENTRAL NERVOUS SYSTEM AND MODULATES THE CHEMOKINE RECEPTORS CCR2 AND CX3CR1
Autor/es:
PERALTA RAMOS, JM; GAVIGLIO, EA; ARROYO, DS; BUSSI, C; RODRIGUEZ-GALAN, MC; IRIBARREN, P
Lugar:
Los Cocos
Reunión:
Congreso; Reunion Anual de la Sociedad Argentina de Inmunología; 2013
Institución organizadora:
SAI
Resumen:
Microglial cells are components of the immune system intrinsic to the central
nervous system and they participate in the response induced by infection,
aseptic inflammation, injury and/or neurodegeneration. These cells migrate to injury
sites in response to chemoattractant agents that are produced under
inflammatory circumstances. This response greatly depends on the expression and
function of chemokine receptors, such as mFPR2, the MCP-1 receptor CCR2 and the
fractalkine receptor CX3CR1 among others. In previous reports we demonstrated
that peptidoglycan (PGN) derived from Staphylococcus aureus, a Toll like
receptor 2 (TLR2) agonist, induced
a considerable increase in the expression of the chemokine receptor mFPR2
in mice microglial cells.
In this work, we evaluate the role of TLR2 in the regulation of the
expression and function of chemokine receptors important in the recruitment of
phagocytes and neuroinflammation such as CCR2 and CX3CR1.
First of all, we observed by flow cytometry that
systemic injection of PGN induces an increased recruitment
of leukocytes to the central nervous system in C57BL/6 mice in comparison to
animals injected with vehicle (p<0.05). On the other hand, in in vitro experiments we assessed either by
RT-PCR and qRT-PCR that direct stimulation of a murine microglial cell line
with PGN induced a gradual decrease of the chemokine receptors CCR2 and CX3CR1
gene expression throughout time (p<0.05).
Our results suggest that stimulation of TLR2 would induce recruitment of
leukocytes to the central nervous system and regulation of chemokine receptors in
microglial cells, which are key in the neuroinflammatory response. However, it
is necessary to further investigate the precise mechanisms underlying this
modulation and which consequences would have over this response.