CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Transcriptional regulation of StarD7 gene by Steroidogenic Factor 1
Autor/es:
RENA VIVIANA; ANGELETTI SOFÍA; PANZETTA-DUTARI GRACIELA; GENTI-RAIMONDI SUSANA
Lugar:
Rosario. Argentina
Reunión:
Congreso; XLII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2006
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica
Resumen:
We have previously cloned and characterized a novel gene up-regulated in choriocarcinoma JEG-3 cell line. This gene, denominated StarD7 encodes a protein that belongs to the StAR-related lipids transfer proteins involved in intracellular transport and metabolism of lipids. To understand the molecular mechanisms that regulate the expression of StarD7 gene, we have cloned and characterized the 5´flanking region of the gene. Transient transfections of several 5´deleted StarD7-promoter-firefly luciferase constructs into JEG-3 and COS-7 cells suggest that the -312/+157 region contains the gene minimal promoter. In addition, sequence analysis of a 1,6 kb gene fragment revealed the presence of a TATA-less promoter as well as multiple regulatory motifs, including one steroidogenic factor 1 (SF-1) binding site at -796/-784 and three cAMPresponsive binding elements at -512/-509, -465/-462 and -115/-111. Electrophoretic mobility shift assays using nuclear proteins from JEG-3 show DNA-protein specific complexes. Co-transfection experiments of JEG-3 and COS-7 cell lines demonstrated that the human StarD7 promoter is activated by SF-1 transcription factor and this effect is increased by cAMP. These studies provide novel insights into the regulation of the human StarD7 gene at the transcriptional level by SF-1