LRP1 EXPRESSION AND FUNCTION IN HUMAN BLOOD PERIPHERAL MONOCYTE (BPM) SUBPOPULATIONS.

FERRER D; JALDIN-FINCATI JR; SÁNCHEZ MC; CHIABRANDO GA

Mendoza

Congreso; XLVIII Reunion Anual SAIB; 2012

SAIB

Alpha2-Macroglobulin (2M*) is recognized by LDL receptor-related protein 1 (LRP1), and induces the cell migration of macrophage. BPM are involved in the development of atherosclerosis. In human, at least three subpopulations of BPM may be identified (classical, intermediate and non-classical). LRP1 it has been associated with atherosclerotic processes. The LRP1 expression and function in BPM subpopulations is not well established. By flow cytometry and confocal microscopy we characterized the LRP1 expression in BPM subpopulations. By two-dimensional wound scratch assay we examined the cell migration of each BPM subpopulation isolated by cell sorter and cultured in presence of 2M*, a LRP1 ligand. LRP1 was three-fold more expressed in classical than in intermediate and non-classical BPM subpopulations. In this subpopulation, LRP1 was mainly localized at perinuclear regions, increasing the cell surface expression under 2M* stimulation for 30 min). In this time, the classical BPM also formed cellular protrusions with accumulation of F-actin and LRP1, which correlated with an increased cell migration. Thus, our data demonstrate classical BPM subpopulation are induce to migrate by the 2M*/LRP1 interaction, which may be involved with the development of atherosclerosis.