Matrix metalloproteinases inducer (EMMPRIN/CD147) expression on B cells of healthy donors and chronic lymphoid leukemia

RODRIGUEZ CM; GILARDONI M; SASTRE D; HELLER V; RAMELLO C; DONADIO AC

Nueva Orleans

Congreso; 27th Annual Meeting of the International Clinical Cytometry Society; 2012

Matrix metalloproteinases (MMPs) play a pivotal role in cell migration and invasion through extracellular matrix. CD147, a cell surface glycoprotein, stimulates production of MMPs on tumor and stromal cells. In this study, we analyzed the CD147 and MMP9 expression in B lymphocytes of healthy donors (HD B cell) and neoplastic B lymphocytes (B-CLL) of patients with Chronic Lymphocytic Leukemia (CLL) and their regulation through B-CLL-fibroblasts (Fb) contact (co-culture) by Flow Cytometry. CD147 median fluorescence intensity (MFI) range in HD B cell (21,6 - 63,7) allowed us to separate CLL in low MFI (19,1 ± 3,2; n=3), middle MFI (42,9 ± 12,5; n=18) and high MFI (77,7 ± 8,3; n=7). CD147 expression was significantly higher in high MFI CLL group compared with moderate (p