CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Spleen dendritic cells from old mice generate a low cytotoxic response against ova in young hosts
Autor/es:
ZACCA, ESTEFANÍA; CRESPO, MARÍA INÉS; MALETTO, BELKYS; PISTORESI, MARÍA CRISTINA; MORÓN, GABRIEL
Lugar:
Buenos Aires
Reunión:
Congreso; FAIC - First French-Argentine Immunology Congress; 2010
Resumen:
During aging, B and T cells manifest changes that affect their response to antigens (Ag). However, it is practically unknown how dendritic cells could participate in these changes. Our previous findings show that in spleen of 18-20 month-oldmice (old) there is a lower content of DCs, which have a lower capacity to activate young naïve CD8 T cells than DCs from 3 month-old (young) mice. In this work we analyzed the ability of DCs from old mice to elicit a CTL response in young mice, in order to analyze the ability of DCs independently of the alterations already reported for CD8 T cells during aging. We first analyzed the capacity from old mice to develop a CTL response in vivo. We immunized i.v young and old mice with 2.5x10e9 latex beads coupled to OVA + PolyU/DOTAP and 7 days later, CTL response was assessed by an in vivo assay. Immunized mice were injected i.v with equal proportions of CFSE-labeled syngeneic spleen cell populations (a control cell population labeled with 0.5 µM CFSE and an OVA257-264 peptide-loaded cell population labeled with 3µM CFSE). One day after injection cytotoxicity was assessed by FACS analysis on total spleen cells. We found that old mice developed a significant lower CTL response against OVA than young mice (4.7±1.5 vs 84±10%, respectively, p