CpG-ODN induces, in vitro and in vivo, myeloid Gr1+CD11b+ cells and suppressor funcctions in young and aged mice.

HARMAN M; RANOCCHIA R; GORLINO C; MALETTO B; MORÓN G; PISTORESI M.C

Buenos Aires

Congreso; First French-Argentine Immunology Congress (FAIC)-LVIII Reunión Científica de la Sociedad Argentina de Inmunología; 2010

SOCIEDAD ARGENTINA DE INMUNOLOGIA

Besides the ability of CpG-ODN to induce in aged (18 months:18m) and young (3 months:3m) BALB/c mice a powerful Th1 immune response, we showed that in vitro CpG-ODN is able to stimulate both, arginase and inducible nitric oxide synthase (iNOS) in macrophages. In the present study, we investigate the regulatory ability of CpG-ODN in vitro and in vivo in 3m and 18m mice and focused on the role of L-arginine metabolism. Bone marrow (BM) cells from 3m and 18m mice were incubated with GM-CSF (3–4 days), resulting in similar percentage of GR1+CD11b+ myeloid cells (3m:50±4%, 18m:55±5%). The treatment of BM-derived myeloid cells with CpG-ODN plus IFN-γ induces arginase activity and nitric oxide (NO) production in both mice ages (p