Candida albicans INDUCES THE ACTIVATION OF THE ARGINASE PATHWAY IN THE HUMAN MONOCYTE CELL LINE (U937)

ICELY, PA; RENNA, MS; SOTOMAYOR, CE

Córdoba. Argentina

Congreso; 1º REUNION INTERNACIONAL DE CIENCIAS FARMACÉUTICAS- RICiFA; 2010

Universidad Nacional Cordoba. Universidad Nacional Rosario

The phagocytes including Monocytes and Macrophages, play an important role in the defence mechanism against the infection with C.albicans. In the metabolism of the L-argnine, the balance between the activation of the inducible Nitric Oxide Sintetase(iNOS) pathway and the arginase pathway, promotes in these cells two alternative states that have been associated with different immune response in the host. The “in vitro” host-pathogen models are a powerfull tool to explore particular interaction and their modulation by therapeutic agents. In the present work we evaluated the activation of L-argnine pathway in a human monocyte cell line(U937), in order to validate previous results obtained with purified human cells(PHC), and to explore the effect of antifungal treatment in the balance of this metabolic pathway. Two antifungal agents used frequently in the treatment of this micosis, Fluconazole and Amphotericine B, were included in this study.