REGULATION OF MICROGLIAL CELL CYTOKINE PRODUCTION BY TYPE 2 CYTOKINES

GAVIGLIO, EA; SORIA, JA; ARROYO, DS; IRIBARREN, P

Buenos Aires

Congreso; First French-Argentine Immunology Congress; 2010

Sociedad Argentina de Inmunología

Inflammation plays an important role in central nervous system (CNS) infection and the progression of neurodegenerative diseases. Microglial cells are phagocytic myeloid cells located in the nervous parenchyma playing a key role in the initiation, progression and resolution of neuroinflammation. It is well known that microglial cells play a key role in mediating inflammatory processes in the CNS, which are associated with various neurodegenerative diseases. Given the potential beneficial effects of anti-inflammatory drugs and cytokines on the treatment and prevention of neurodegenerative diseases, we investigated the role of IL-4 and IL-13 in the regulation of cytokine production by microglial cells. First of all, we evaluated the effects of stimulation of mouse microglial cell lines with IL-4, IL-13 or INF-gamma, on the production of TNF alpha, IL-10 and IL-27. Both, IL-4 and IL-13 failed to induce the secretion of TNFalpha or IL-27 (p NS). However, IL-4 induced the secretion of the anti-inflammatory cytokine IL-10. In another set of experiments we tested if IL-4 or IL-13 were capable to inhibit the cytokine production of microglial cells activated by pro-inflammatory molecules, such as lipopolysaccharide (LPS) and INF gamma. We found that IL-4 inhibited the production of IL-6 on LPS-stimulated microglial cells (p