CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
CD4+CD25+Foxp3+ regulatory T cells induced by the immunization with tolerogenic dendritic cells attenuate collagen-indiced arthritis symptoms in DBA/1J mice
Autor/es:
CARRANZA F; FALCON C; CERVI L
Lugar:
BUENOS AIRES
Reunión:
Congreso; FIRST FRENCH-ARGENTINE IMMUNOLOGY CONGRESS (FAIC); 2010
Institución organizadora:
SAI - SAIC
Resumen:
Dendritic cells (DC) are professional antigen-presenting cells that maintain immune tolerance to self-antigens by controlling the pathogenicity of auto-reactive T-cells. These cells can be modified ex vivo to induce tolerogenic function and inhibit inflammatory responses in autoimmune diseases. Previous results show that the treatment of DBA/1J mice bone marrow derived DC with a total extract of the parasite Fasciola hepatica (TE) plus CpG (T/C) induces tolerogeneic properties such as a decrease in pro-inflammatory cytokine production, an increases in anti-inflammatory cytokine production and a high expression of indoleamine 2,3-dioxigenasa (IDO). Furthermore, the immunization of DBA/1J mice with T/C-treated DC was able to ameliorate the symptoms of collagen-induced arthritis (CIA). The aim of this work was to evaluate the involvement of CD4+Foxp3+ regulatory T cells in the mechanism by which the immunization of mice with T/C-treated DC diminishes the symptoms of CIA. The immunization of mice with T/C-treated DC induced an increase in the percentage of CD4+CD25+Foxp3+ T cells, determined by flow citometry in draining lymph nodes (DLN), compare to the control (PBS injected mice). Besides, the recipient mice of T/C-treated DC showed a significant increase in IL-10 production as well as a decrease in INF-ƒ× production detected in the supernatants of DLN of culture compared to the control (p