CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
FB1-INDUCED PHYTOTOXICITY: REDOX STATE OF DIFFERENT INTRACELLULAR COMPARTMENTS AND THEIR CORRELATION WITH AUTOPHAGY
Autor/es:
ENET, ALEJANDRO; LASCANO, H. RAMIRO; OTAIZA GONZÁLEZ, SANTIAGO NICOLÁS; THEUMER, MARTIN GUSTAVO; ROBERT, GERMÁN
Lugar:
Santa Fe
Reunión:
Congreso; XXXIII Argentinian meeting of Plant Physiology (RAFV2021); 2021
Institución organizadora:
Sociedad Argentina de Fisiología Vegetal
Resumen:
Mycotoxin FB1 is animportant pathogenicity factor in the Fusarium verticillioides-plantinteraction and alone induces many of the phytopathological signs observed ininfection. The aim of the work was to correlate the role of autophagy andredox changes in different intracellular compartments in a model of acutephytotoxicity induced by FB1. Arabidopsis Col-0, atg7 mutant seedlings weregrown with FB1 (0-1 µM). At 7 days after planting, cell death was determined byelectrolyte leakage (EL) y and ratiometric redox-sensitive GFP (roGFP)expressed in different compartments (cytoplasm, mitochondrion, peroxisome andchloroplast) of the plant, were observed by confocal microscope and analyzed withFIJI ImageJ software. FB1 induced different EL profile in atg7 with respect toCol-0 (decrease and increase at low and high concentrations of FB1,respectively). Also, a different oxidation level was observed in the cytoplasmin atg7 at 0.1 µM (decrease) and 1 µM (increase) of FB1 with respect to Col-0. Ourpreliminary results suggest that autophagy induces death at low concentrationsof FB1 and inhibition at high concentrations of the mycotoxin. Likewise, celldeath is correlated with a higher level of oxidation in the cellular cytoplasm.