Study of phenotype and dynamic of T-cell mediated antifungal immune in the skin

BURSTEIN VL; MENA, CRISTIAN J.; GRUPPI A; BECCACECE, IGNACIO; ALMEIDA, MARIEL; PRINZ I; GUASCONI, LORENA; CERVI L; CHIAPELLO L S

Córdoba

Congreso; 19th Meeting of Expert in Clinical Mycology-INFOCUS 2021; 2021

INFOCUS Latam ISHAM Working Group y Círculo Médico de Córdoba

The skin is a physical barrier with a dynamic resident immune system that is crucial to control infection, resolve damage or maintain tissue homeostasis. In the last few years it has been discovered that tissue-resident T lymphocytes play a central role in the immunity against pathogens in barrier organs. Dermatophytes are a highly prevalent cause of human disease worldwide, however, the phenotype and dynamic of skin immune cell subsets controlling dermatophyte infection remains poorly understood.We have previously demonstrated that IL-17-mediated immunity (IL-17A and IL-17F) is important in controlling fungal overgrowth in the skin in an experimental model of dermatophytosis in C57BL/6 mice (Microsporum canis or Nannizzia gypsea epicutaneous infection). In this study, we aimed to investigate the immune cell subsets that produce IL-17A in the skin and the role of tissue-resident cells in the antifungal immunity during experimental dermatophytosis with N. gypsea.By employing transgenic IL-17A-GFP-reporter mice and flow cytometry (FC) analysis of epidermal cell suspensions, we observed a significant increase in the frequency of IL-17A-producing CD45+CD3+ T cells at 6 and 8 days of N. gypsea infection in back skin, compared to saline-treated mice (uninfected control, P