PATHWAY ANALYSIS OF CHOLESTERYL ESTER ACCUMULATION EFFECT ON INSULIN RESPONSE IN HL-1 CARDIOMYOCYTES.

CHIABRANDO, GUSTAVO A; REY FEDERICO; ACTIS DATO VIRGINIA; CESCHIN DANILO G

Virtual

Congreso; SAIB - SAMIGE Joint Meeting 2021 on line; 2021

SAIB

The cardiovascular disease (CVD) is a multisystem and multicellular pathology frequently associated with increased levels ofaggregation-prone small LDL particles. Aggregated LDL (aggLDL) internalization promotes intracellular cholesteryl ester(CE) accumulation and the impairment of insulin response and glucose transporter type 4 (GLUT4) activity in cardiomyocytes.Lipid uptake was associated with cardiac dysfunction and cell death. Nevertheless, the link between CE accumulation andinsulin response, cell function and viability in cardiomyocytes is not completely understood yet. The present study wasconducted to determine intracellular pathways that are potentially regulated by cholesterol accumulation in heart. For this, weidentify gene expression profiles by bulk transcriptome analysis in isolated RNA samples from cardiomyocyte-derived HL-1cells exposed to aggLDL 100 µg/ml for 8 h and then stimulated or not with insulin 100 nM for 2 h. Considering a fold change(FC)>2 and p