CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
artículos
Título:
A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina
Autor/es:
ANDREA G. ALBARRACÍN ORIO, PAULO R. CORTES, MIGUEL TREGNAGHI, GERMAN E. PIÑAS, ARGENTINEAN NETWORK PNEUMOCOCCUS STUDY GROUP AND JOSÉ R. ECHENIQUE
Revista:
JOURNAL OF MEDICAL MICROBIOLOGY
Editorial:
SGM
Referencias:
Lugar: Reading; Año: 2008 p. 992 - 999
ISSN:
0022-2615
Resumen:
The penicillin-resistant Spain9V-3 clone of Streptococcus pneumoniae is widespread and
presents different serotype variants originating from recombination of the capsular genes. In this
work, the genetic relatedness of 29 invasive pneumococci isolated from the central region of
Argentina (Cordoba, Buenos Aires, Santa Fe and La Pampa provinces) was assessed by
multilocus sequence typing (MLST). All of the penicillin-non-susceptible isolates studied (21/29)
belonged to a serotype 14 variant of the Spain9V-3 clone. This clone was predominant,
suggesting that it was responsible for the penicillin resistance spread in this region. Interestingly,
this serotype 14 variant (named Cordoba S14V) could be differentiated from the European one by
its pbp1a gene, suggesting a different recombinational replacement of the capsular genes. The
putative recombination sites were analysed, resulting in the proximal crossover point being clearly
localized in the spr0309 gene, with the distal site restricted to the recU gene, confirming a
different recombination event. Analysis of the dexB, cpsB, aliA and pbp1a genes from these
strains showed a high similarity with the corresponding genes of the Spain14-5 clone, suggesting
that the capsular genes were provided by this international clone. Analysis of the genetic
polymorphisms of the pbp1a (nt 14731922) and spr0309 (nt 1790) genes is proposed as an
epidemiological tool to help recognize the Cordoba S14V of the Spain9V-3 clone. On the other
hand, BOX-repeat-based PCR and MLST analyses of serotype 14 strains revealed a divergent
epidemiology of the Cordoba S14V, suggesting a non-recent dissemination in the paediatric
population. It is suggested that this molecular epidemiology work will be a reference for
monitoring the evolution of S14Vs of Spain9V-3, the emergence of new clones and the impact of
pneumococcal vaccination programmes in Argentina.9V-3 clone of Streptococcus pneumoniae is widespread and
presents different serotype variants originating from recombination of the capsular genes. In this
work, the genetic relatedness of 29 invasive pneumococci isolated from the central region of
Argentina (Cordoba, Buenos Aires, Santa Fe and La Pampa provinces) was assessed by
multilocus sequence typing (MLST). All of the penicillin-non-susceptible isolates studied (21/29)
belonged to a serotype 14 variant of the Spain9V-3 clone. This clone was predominant,
suggesting that it was responsible for the penicillin resistance spread in this region. Interestingly,
this serotype 14 variant (named Cordoba S14V) could be differentiated from the European one by
its pbp1a gene, suggesting a different recombinational replacement of the capsular genes. The
putative recombination sites were analysed, resulting in the proximal crossover point being clearly
localized in the spr0309 gene, with the distal site restricted to the recU gene, confirming a
different recombination event. Analysis of the dexB, cpsB, aliA and pbp1a genes from these
strains showed a high similarity with the corresponding genes of the Spain14-5 clone, suggesting
that the capsular genes were provided by this international clone. Analysis of the genetic
polymorphisms of the pbp1a (nt 14731922) and spr0309 (nt 1790) genes is proposed as an
epidemiological tool to help recognize the Cordoba S14V of the Spain9V-3 clone. On the other
hand, BOX-repeat-based PCR and MLST analyses of serotype 14 strains revealed a divergent
epidemiology of the Cordoba S14V, suggesting a non-recent dissemination in the paediatric
population. It is suggested that this molecular epidemiology work will be a reference for
monitoring the evolution of S14Vs of Spain9V-3, the emergence of new clones and the impact of
pneumococcal vaccination programmes in Argentina.9V-3 clone. This clone was predominant,
suggesting that it was responsible for the penicillin resistance spread in this region. Interestingly,
this serotype 14 variant (named Cordoba S14V) could be differentiated from the European one by
its pbp1a gene, suggesting a different recombinational replacement of the capsular genes. The
putative recombination sites were analysed, resulting in the proximal crossover point being clearly
localized in the spr0309 gene, with the distal site restricted to the recU gene, confirming a
different recombination event. Analysis of the dexB, cpsB, aliA and pbp1a genes from these
strains showed a high similarity with the corresponding genes of the Spain14-5 clone, suggesting
that the capsular genes were provided by this international clone. Analysis of the genetic
polymorphisms of the pbp1a (nt 14731922) and spr0309 (nt 1790) genes is proposed as an
epidemiological tool to help recognize the Cordoba S14V of the Spain9V-3 clone. On the other
hand, BOX-repeat-based PCR and MLST analyses of serotype 14 strains revealed a divergent
epidemiology of the Cordoba S14V, suggesting a non-recent dissemination in the paediatric
population. It is suggested that this molecular epidemiology work will be a reference for
monitoring the evolution of S14Vs of Spain9V-3, the emergence of new clones and the impact of
pneumococcal vaccination programmes in Argentina.pbp1a gene, suggesting a different recombinational replacement of the capsular genes. The
putative recombination sites were analysed, resulting in the proximal crossover point being clearly
localized in the spr0309 gene, with the distal site restricted to the recU gene, confirming a
different recombination event. Analysis of the dexB, cpsB, aliA and pbp1a genes from these
strains showed a high similarity with the corresponding genes of the Spain14-5 clone, suggesting
that the capsular genes were provided by this international clone. Analysis of the genetic
polymorphisms of the pbp1a (nt 14731922) and spr0309 (nt 1790) genes is proposed as an
epidemiological tool to help recognize the Cordoba S14V of the Spain9V-3 clone. On the other
hand, BOX-repeat-based PCR and MLST analyses of serotype 14 strains revealed a divergent
epidemiology of the Cordoba S14V, suggesting a non-recent dissemination in the paediatric
population. It is suggested that this molecular epidemiology work will be a reference for
monitoring the evolution of S14Vs of Spain9V-3, the emergence of new clones and the impact of
pneumococcal vaccination programmes in Argentina.spr0309 gene, with the distal site restricted to the recU gene, confirming a
different recombination event. Analysis of the dexB, cpsB, aliA and pbp1a genes from these
strains showed a high similarity with the corresponding genes of the Spain14-5 clone, suggesting
that the capsular genes were provided by this international clone. Analysis of the genetic
polymorphisms of the pbp1a (nt 14731922) and spr0309 (nt 1790) genes is proposed as an
epidemiological tool to help recognize the Cordoba S14V of the Spain9V-3 clone. On the other
hand, BOX-repeat-based PCR and MLST analyses of serotype 14 strains revealed a divergent
epidemiology of the Cordoba S14V, suggesting a non-recent dissemination in the paediatric
population. It is suggested that this molecular epidemiology work will be a reference for
monitoring the evolution of S14Vs of Spain9V-3, the emergence of new clones and the impact of
pneumococcal vaccination programmes in Argentina.dexB, cpsB, aliA and pbp1a genes from these
strains showed a high similarity with the corresponding genes of the Spain14-5 clone, suggesting
that the capsular genes were provided by this international clone. Analysis of the genetic
polymorphisms of the pbp1a (nt 14731922) and spr0309 (nt 1790) genes is proposed as an
epidemiological tool to help recognize the Cordoba S14V of the Spain9V-3 clone. On the other
hand, BOX-repeat-based PCR and MLST analyses of serotype 14 strains revealed a divergent
epidemiology of the Cordoba S14V, suggesting a non-recent dissemination in the paediatric
population. It is suggested that this molecular epidemiology work will be a reference for
monitoring the evolution of S14Vs of Spain9V-3, the emergence of new clones and the impact of
pneumococcal vaccination programmes in Argentina.14-5 clone, suggesting
that the capsular genes were provided by this international clone. Analysis of the genetic
polymorphisms of the pbp1a (nt 14731922) and spr0309 (nt 1790) genes is proposed as an
epidemiological tool to help recognize the Cordoba S14V of the Spain9V-3 clone. On the other
hand, BOX-repeat-based PCR and MLST analyses of serotype 14 strains revealed a divergent
epidemiology of the Cordoba S14V, suggesting a non-recent dissemination in the paediatric
population. It is suggested that this molecular epidemiology work will be a reference for
monitoring the evolution of S14Vs of Spain9V-3, the emergence of new clones and the impact of
pneumococcal vaccination programmes in Argentina.pbp1a (nt 14731922) and spr0309 (nt 1790) genes is proposed as an
epidemiological tool to help recognize the Cordoba S14V of the Spain9V-3 clone. On the other
hand, BOX-repeat-based PCR and MLST analyses of serotype 14 strains revealed a divergent
epidemiology of the Cordoba S14V, suggesting a non-recent dissemination in the paediatric
population. It is suggested that this molecular epidemiology work will be a reference for
monitoring the evolution of S14Vs of Spain9V-3, the emergence of new clones and the impact of
pneumococcal vaccination programmes in Argentina.9V-3 clone. On the other
hand, BOX-repeat-based PCR and MLST analyses of serotype 14 strains revealed a divergent
epidemiology of the Cordoba S14V, suggesting a non-recent dissemination in the paediatric
population. It is suggested that this molecular epidemiology work will be a reference for
monitoring the evolution of S14Vs of Spain9V-3, the emergence of new clones and the impact of
pneumococcal vaccination programmes in Argentina.9V-3, the emergence of new clones and the impact of
pneumococcal vaccination programmes in Argentina.