CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
artículos
Título:
Systemic IL-12 burst expands intestinal T lymphocyte subsets bearing the alpha4beta7 integrin in mice
Autor/es:
PEDROTTI LP, BARRIOS BE, MACCIO MARETTO L, BENTO A, SENA AA, RODRIGUEZ GALAN MC, CALIXTO JB, CORREA SG
Revista:
EUROPEAN JOURNAL OF IMMUNOLOGY
Editorial:
WILEY-V C H VERLAG GMBH
Referencias:
Lugar: Weinheim; Año: 2016 vol. 46 p. 70 - 80
ISSN:
0014-2980
Resumen:
The intestinal immune system is complex and displays unique anatomic and functionalcharacteristics. Numerous subsets of immune cells are located beneath the epithelialbarrier and their activity is highly regulated. Using hydrodynamic shear of IL-12 cDNAto achieve systemic expression of IL-12 we evaluated the effect of the transient burst ofthe cytokine on the activation status of T cells from Peyer`s patches (PP), mesentericlymph nodes (MLN) and colonic lamina propria (LP). Following systemic IL-12 release,intestinal T lymphocytes became activated, exhibiting a CD44high CD62L- phenotype.After 5 days of the cytokine burst, the frequency of α4β7+ CD4+ and CD8+ cellsincreased, and CD8+ α4β7+ cells mainly expressed the transcription factor T-bet, acritical regulator of the Th1 differentiation program. The increment of the homingmolecule involved the IL-12 receptor- signal transducer and activator oftranscription (STAT)-4 axis and was independent of IFNγ, IL-4, IL-10 and TNFαsignals. Moreover, the IL-12 priming exacerbated the outcome of acute dextran sodiumsulphate (DSS)-colitis modifying the effector response triggered by the luminal stimuli.Together our findings demonstrate that systemic polarizing signals effectively couldexpand the amount of effector cells able to home to the LP and contribute toinflammatory responses.