DEIMINATION LEVEL AND PEPTIDYL ARGININE DEIMINASE 2 EXPRESSION IS ELEVATED IN ASTROCYTES WITH INCREASED INCUBATION TEMPERATURE.

ALGECIRAS M; BHATTACHARYA SK; SERRA H.M

JOURNAL OF NEUROSCIENCE RESEARCH

WILEY-LISS, DIV JOHN WILEY & SONS INC

Lugar: New York; Año: 2015

0360-4012

Astrocytes respond to environmental cues includingchanges in temperatures. Increased deimination, observed in many progressiveneurological diseases are thought to be contributed by astrocytes. Wedetermined the level of deimination and expression of peptidylargininedeiminase 2 (PAD2) in isolated primary astrocytes in response to changes oneither side (31°C as well as 41°C) of the optimal temperature (37°C). Weinvestigated changes in the astrocytes using a number of established markersand also accounted for cell death using theCelltiter blue assay. We foundincreased expression of GFAP, ALDH1L1 and J1-31 due to increased temperatureincubation and increased expression of TSP1, S100B and AQP4 due to decreasedtemperature incubation from optimal temperature suggesting activation ofdifferent biochemical pathways in astrocytes associated with differentincubation temperatures. Mass spectrometric analyses support such trends. PAD2level was increased only due to increased incubation temperature with acommensurate increased level of deimination. Interestingly actin cytoskeletonand IsoLGE4, a lipid peroxidase modification also showed an increase withhigher incubation temperature. Taken together these results suggest thattemperature as an environmental cue activates astrocytes in a different manneron either side of optimal temperature and that increase in deimination isassociated only with the higher temperature side of the spectrum.