PROTEOMIC ANALYSIS OF CLIMATIC KERATOPATHY DROPLETS.

MICHELLE MENEGAY,; DEMIA LEE; KHALID F. TABBARA; THAMARA A. CAFARO; JULIO A. URRETS-ZAVALÍA; HORACIO M. SERRA; SANJOY K. BHATTACHARYA.

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE

Año: 2008 vol. 49 p. 2829 - 2837

0146-0404

AbstractPurpose. To identify the proteins in the corneal droplets of climatic dropletkeratopathy (CDK), a disease that results in the formation of droplets on thecornea. Progressive accumulation of droplets in CDK leads to visual loss.Methods. Proteomic mass spectrometry of the CDK specimens was performedafter fractionation of proteins on a 4-20% SDS-PAGE. Droplets were derivedfrom two human donors. Immunohistochemistry using antibodies were performedto confirm the presence of identified proteins on donor tissues from CDK patientsand controls.Results. Proteomic analyses revealed identification of 105 proteins in CDKspecimens. Immunohistochemical analyses confirmed localization of Annexin A2and Glyceraldehyde 3-dehydrogenase (GAPDH), protein identified by proteomicanalyses in CDK specimens. Identified proteins were subjected to analysesutilizing Kyoto encyclopedia of genes and genomes (KEGG) database thatshowed a few biochemical pathways more frequent for the identified proteins.Conclusion. About 105 proteins have been identified in CDK specimens and asubset of them has been confirmed using immunohistochemistry. A number ofthese bear the potential of fibril or deposit formation.