Inhibitory effect of cyclic terpenes (limonene, menthol, menthone and thymol) on Fusarium verticillioides MRC 826 growth and its fumonisin B1 biosynthesis.

DAMBOLENA JS; LÓPEZ AG; ZYGADLO JA; CÁNEPA MC; THEUMER MG; RUBINSTEIN HR

TOXICON

Elsevier

Año: 2008 vol. 51 p. 37 - 44

0041-0101

The minimum inhibitory concentration (MIC) of cyclic terpenes (limonene, menthol, menthone and thymol) against Fusarium verticillioides MRC 826 was assessed by using the semisolid agar antifungal susceptibility (SAAS) technique.  Limonene, menthol, menthone and thymol were evaluated at final concentrations of 25, 50, 75, 150, 200, 250, 500 and  1000 mL/L of culture medium. Limonene and thymol showed the highest inhibitory effects on F. verticillioides  development. Thus, the effects of monoterpenes on fumonisin B1 (FB1) biosynthesis were evaluated by using corn grain  (Zea mays) as substrate. The monoterpenes were inserted on maize 1 day before inoculation with a conidial suspension of  F. verticillioides to give final concentrations of 75 ppm. At this concentration, thymol was the most active inhibitor on FB1 biosynthesis.  Fusarium verticillioides MRC 826 was assessed by using the semisolid agar antifungal susceptibility (SAAS) technique.  Limonene, menthol, menthone and thymol were evaluated at final concentrations of 25, 50, 75, 150, 200, 250, 500 and  1000 mL/L of culture medium. Limonene and thymol showed the highest inhibitory effects on F. verticillioides  development. Thus, the effects of monoterpenes on fumonisin B1 (FB1) biosynthesis were evaluated by using corn grain  (Zea mays) as substrate. The monoterpenes were inserted on maize 1 day before inoculation with a conidial suspension of  F. verticillioides to give final concentrations of 75 ppm. At this concentration, thymol was the most active inhibitor on FB1 biosynthesis. Fusarium verticillioides MRC 826 was assessed by using the semisolid agar antifungal susceptibility (SAAS) technique.  Limonene, menthol, menthone and thymol were evaluated at final concentrations of 25, 50, 75, 150, 200, 250, 500 and  1000 mL/L of culture medium. Limonene and thymol showed the highest inhibitory effects on F. verticillioides  development. Thus, the effects of monoterpenes on fumonisin B1 (FB1) biosynthesis were evaluated by using corn grain  (Zea mays) as substrate. The monoterpenes were inserted on maize 1 day before inoculation with a conidial suspension of  F. verticillioides to give final concentrations of 75 ppm. At this concentration, thymol was the most active inhibitor on FB1 biosynthesis.