CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
artículos
Título:
"Galectin-1 functions as a Th2 cytokine that selectively induces Th1 apoptosis and promotes Th2 function"
Autor/es:
MOTRAN, CLAUDIA C; MOLINDER, KAREN; LIU, SCOT; POIRIER, F; MICELI, MC
Revista:
EUROPEAN JOURNAL OF IMMUNOLOGY
Referencias:
Año: 2008
ISSN:
0014-2980
Resumen:
Abstract Galectin-1 has been implicated in regulating T cell survival, function and Th1/Th2 balance in several mouse models, though the molecular and cellular basis of its immunomodulatory activity has not been completely elucidated. Therefore, we examined galectin-1 expression and activity within differentiated murine Th1 and Th2 subsets. While galectin-1 specifically bound to both T cell subsets, Th1 and Th2 T cells expressed distinct constellations of galectin-1 reactive epitopes and were differentially responsive to galectin-1 exposure. Indeed, Th1 cells were more susceptible to galectin-1 induced death than Th2 cells. Th2 protection from apoptosis was correlated with expression of antiapoptotic galectin-3. Further, galectin-1 diminished TCR-induced type 1 Th1 cytokine production, while it promoted TCR-induced type 2 cytokine production by Th2 cells. Differentiated Th2 cells constitutively expressed high levels of galectin-1, whereas comparable levels of galectin-1 in Th1 cells were only observed after re-stimulation. Coculturing galectin-1-/- and galectin-1+/+ Th1 and Th2 T cells demonstrated that Th2- derived galectin-1 induced Th1 apoptosis and antagonized Th1 cytokine production, whereas Th1-derived galectin-1 promoted Th2 cytokine production. These studies identify galectin-1 as a cross-regulatory cytokine that selectively antagonizes Th1 function and survival, while promoting TCR-induced Th2 cytokine production.-/- and galectin-1+/+ Th1 and Th2 T cells demonstrated that Th2- derived galectin-1 induced Th1 apoptosis and antagonized Th1 cytokine production, whereas Th1-derived galectin-1 promoted Th2 cytokine production. These studies identify galectin-1 as a cross-regulatory cytokine that selectively antagonizes Th1 function and survival, while promoting TCR-induced Th2 cytokine production.