CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
artículos
Título:
Extracellular lipase of Candida albicans mediate cell injury and steatosis in vitro
Autor/es:
PARAJE MG; CORREA SG; RENNA MS; THEUMER M; SOTOMAYOR CE
Revista:
Can. J Microbiol.
Referencias:
Año: 2008
Resumen:
Virulence depends on opposing reactions between  host and pathogen and is intrinsically linked to the host immune-status. Virulence factors rely upon microbial attributes that mediate cell damage. While the activity of several C. albicans hydrolytic enzymes is well characterized, the biological role of lipases is uncertain. In this report, we identified, isolated, and characterized a C. albicans 70 kDa lipase, that exhibited maximal activity at physiological pH and temperature.  We  evaluated the ability of C. albicans lipase to interact with two types of mammalian host cells: macrophages (Mfs), as crucial immune effector cells involved in the fungal control, and hepatocytes (Hps), as example of  parenchymal cells  compromised during  fungal dissemination. Herein we demonstrate, for the first time, that an extracellular lipase released by C. albicans directly induced cytotoxicity  and  promoted the deposition of lipid droplets in the cytoplasm of Mfs and Hps.