. Modulation of Dendritic Cell Maturation by Fasciola hepatica: Implications of glycans and mucins for Vaccine Development

NOYA V; RODRIGUEZ ERNESTO; . CERVI L.; GIACOMINI CECILIA; NATALIE BROSSARD; CAROLINA CHIALE; CARLOS CARMONA; TERESA FREIRE

Journal of Vaccines & Vaccination

OMICS Publlishing group

Año: 2014 vol. 5 p. 4 - 8

2157-7560

Fasciola hepatica is a worldwide distributed helminth pathogen that causes great economic losses in sheep and cattle. This parasite is able to regulate the host immune response, producing high levels of IL-5 and low levels of IFNγ, as well as modulating the function of dendritic cells (DCs), mast cells or macrophages, among others. Moreover, TLR-mediated maturation of DCs can be suppressed by F. hepatica derived components. Here, we investigated the role of glycans in the modulation of LPS-induced maturation of DCs, as well as in the production of IL-5 and IFNγ by splenocytes from infected mice. We show that F. hepatica induces the recruitment to the peritoneum of semi-matured DCs, as judged by a down-regulation of MHC class II molecule expression and an increase of CD80 and CD86 expression of DCs in the peritoneum of infected animals. Furthermore, we provide evidence indicating that glycan structures from F. hepatica are responsible, at least in part, for inhibiting LPSinduced DC maturation and production of IFNγ by splenocytes from infected animals. On the other hand, we show that a mucin-like non-glycosylated peptide highly expressed in NEJ (Fhmuc) is able to synergize with LPS in inducing DC-maturation, and that it induces a T cell response specific for F. hepatica, both alone or in combination with DCs. Our data highlight the role of F. hepatica glycans in modulating the host immune response and might contribute to the design of vaccines against fasciolosis.