Prostate epithelial cells can act as early sensors of infection by up-regulating TLR4 expression and proinflammatory mediators upon LPS stimulation

GATTI G; RIVERO V; MOTRICH RD; MACCIONI M

JOURNAL OF LEUKOCYTE BIOLOGY

Año: 2006 vol. 79 p. 989 - 998

0741-5400

Despite the prevalence of prostate disease, little is known about the immunobiology of the prostate and its contribution to disease.  The main goal of this work was to investigate how prostate epithelial cells deal with inflammatory stimuli.  To this aim, we stimulated a rat prostate epithelial cell line (MAT-LU) or rat primary epithelial cells with lipopolysaccharide.  Prostate epithelial cells constitutively express significant levels of TLR4 and CD14 mRNA.  TLR2 transcription could also been demonstrated, suggesting that these cells could recognize a broader spectrum of microbial molecular patterns. TLR4, TLR2 and CD14 proteins were also detected, although not at the cell surface but intracellularly.  Prostate epithelial cells not only express these receptors, but they are able to respond to LPS.  LPS-stimulated MAT-LU cells activate NF-kB transcription factor, induce the expression of iNOS and secrete NO. Evenmore, numerous chemokine genes are either up-regulated or induced in this response. Our results clearly demonstrate that prostate epithelial cells are fully competent to respond.  The fact that they express TLR4 and TLR2 intracellularly suggests the presence of regulatory mechanisms, that once overcome, could turn these cells into active players of the innate immunity, capable of initiating an inflammatory response.