Presence of neutrophil bearing antigen in lymphoid organs of immune mice.

MALETTO B; RÓPOLO, A; ALIGNANI, D; LISCOVSKY, M; RANOCCHIA, R; MORÓN G; PISTORESI-PALENCIA MC

BLOOD, THE JOURNAL OF THE AMERICAN SOCIETY OF HEMATOLOGY - PRINT

Año: 2006 vol. 108 p. 3094 - 3102

0006-4971

Neutrophils play a crucial early role during the innate response, but little is known about their possible contribution when an adaptative immune response is installed. A robust neutrophilia and a Th1 immune response are present after immunization with Complete Freund’s Adjuvant (CFA). We show that when FITC-labeled OVA was injected into the footpad of OVA/CFA immunized mice, the main OVA-FITC+ cells recruited in draining popliteal lymph nodes (LN) were neutrophils, with most of them arriving at the LN by means of lymphatic vessels. The development of this OVA-FITC+ neutrophil influx requires an immune response against OVA. The OVA-FITC+ neutrophils present in LN displayed mainly intracellular TNF-á and their depletion resulted in an increase in the specific IL-5 levels. These data provide new evidence about the role played by neutrophils+ cells recruited in draining popliteal lymph nodes (LN) were neutrophils, with most of them arriving at the LN by means of lymphatic vessels. The development of this OVA-FITC+ neutrophil influx requires an immune response against OVA. The OVA-FITC+ neutrophils present in LN displayed mainly intracellular TNF-á and their depletion resulted in an increase in the specific IL-5 levels. These data provide new evidence about the role played by neutrophils+ neutrophil influx requires an immune response against OVA. The OVA-FITC+ neutrophils present in LN displayed mainly intracellular TNF-á and their depletion resulted in an increase in the specific IL-5 levels. These data provide new evidence about the role played by neutrophils+ neutrophils present in LN displayed mainly intracellular TNF-á and their depletion resulted in an increase in the specific IL-5 levels. These data provide new evidence about the role played by neutrophilsá and their depletion resulted in an increase in the specific IL-5 levels. These data provide new evidence about the role played by neutrophils in vivo in adaptative immunity.in adaptative immunity.