Effects of the organophosphate insecticides phosmet and chlorpyrifos on trophoblast 3 JEG-3 cell death, proliferation and inflammatory molecule production

GUIÑAZÚ NATALIA; RENA VIVIANA; GENTI DE RAIMONDI SUSANA; RIVERO VIRGINIA; MAGNAR

TOXICOLOGY IN VITRO : AN INTERNATIONAL JOURNAL PUBLISHED IN ASSOCIATION WITH BIBRA.

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2012 vol. 3 p. 406 - 413

0887-2333

Epidemiological data have associated environmental organophosphate insecticide (OP) exposure during pregnancy with fetal growth deficits. To better understand OP injury that may adversely affect pregnancy, we used the JEG-3 choriocarcinoma cell line, which provide a recognized in vitro model to studyplacental function. The effects of the OP phosmet (Pm) and chlorpyrifos (Cp) on JEG-3 cells viability, proliferation, cell cycle and inflammatory molecule production were evaluated. Both insecticides affected cellular viability in a concentration- and time-dependent manner, inducing apoptosis and decreasing[3H]-thymidine incorporation. However, only Pm reduced DNA synthesis independently of cellular death 30 and decreased the cell percentage at the S-phase. Unlike apoptosis, TNFa production varied with the concentration tested, suggesting that other TNFa independent mechanisms might trigger cell death. Noinduction of the inflammatory molecule nitric oxide was detected. The mRNA levels of pro-inflammatoryIL-6, IL-17 and the anti-inflammatory IL-13 cytokines were differentially modulated. These findings show that Pm and Cp generate a specific toxicity signature, altering cell viability and inducing an inflammatory cytokine profile, suggesting that trophoblasts may represent a possible target for OP adverse effects