CIBICI 14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
artículos
Título:
Structural organization of the mitochondrial DNA control in Aedes aegypti
Autor/es:
RONDAN DUEÑAS, JUAN C; GARDENAL, CRISTINA N; ALBRIEU LLINÁS, GUILLERMO; PANZETTA-DUTARI, GRACIELA M
Revista:
GENOME
Editorial:
Research Press National Research Council of Canada
Referencias:
Lugar: Canada; Año: 2006 vol. 49 p. 931 - 937
ISSN:
0831-2796
Resumen:
The complete A+T - rich region of Aedes aegypti mitochondrial DNA has been cloned and sequenced. In
Argentinean populations of the species, a polymorphism in the length of the amplified fragment was observed. Nucleotide
sequence comparison of the shortest and longest A+T - rich amplified fragments detected revealed the presence of
2 types of tandemly repeated blocks. The size variation observed in natural populations is mainly due to the presence
of a variable number of a 181 bp tandem repeat unit, located toward the 12S rRNA gene end. The size of the longest
A+T - rich region was of 2070 bp, representing the largest control sequence reported for any mosquito species. Few
relevant short blocks of primary-sequence similarity conserved in the control region of mosquitoes and other insects
were detected scattered throughout the whole region. Five putative stem-loop secondary structures were found, one of
them flanked by conserved sequences described in other insects. Our results suggest that there are no universal models
of structurefunction relations in the control region of insect mtDNA. In addition, we identified a short A+T - rich
variable segment in the Ae. aegyti control region that would be suitable for population genetic studies.Aedes aegypti mitochondrial DNA has been cloned and sequenced. In
Argentinean populations of the species, a polymorphism in the length of the amplified fragment was observed. Nucleotide
sequence comparison of the shortest and longest A+T - rich amplified fragments detected revealed the presence of
2 types of tandemly repeated blocks. The size variation observed in natural populations is mainly due to the presence
of a variable number of a 181 bp tandem repeat unit, located toward the 12S rRNA gene end. The size of the longest
A+T - rich region was of 2070 bp, representing the largest control sequence reported for any mosquito species. Few
relevant short blocks of primary-sequence similarity conserved in the control region of mosquitoes and other insects
were detected scattered throughout the whole region. Five putative stem-loop secondary structures were found, one of
them flanked by conserved sequences described in other insects. Our results suggest that there are no universal models
of structurefunction relations in the control region of insect mtDNA. In addition, we identified a short A+T - rich
variable segment in the Ae. aegyti control region that would be suitable for population genetic studies.Ae. aegyti control region that would be suitable for population genetic studies.
