CIBICI   14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
artículos
Título:
Immunization with crude antigens plus aluminium hydroxide protects cattle from Fasciola hepatica infection
Autor/es:
GUASCONI, L; SERRADELL, MC; BORGONOVO, J; GARRO, AP; VARENGO, H; CAFFE, G; MASIH, DT
Revista:
JOURNAL OF HELMINTHOLOGY
Editorial:
CAMBRIDGE UNIV PRESS
Referencias:
Año: 2011 p. 1 - 6
ISSN:
0022-149X
Resumen:
The ability of total homogenate (TH) of Fasciola hepatica conjugated with
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
The ability of total homogenate (TH) of Fasciola hepatica conjugated with
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
The ability of total homogenate (TH) of Fasciola hepatica conjugated with
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
The ability of total homogenate (TH) of Fasciola hepatica conjugated with
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
The ability of total homogenate (TH) of Fasciola hepatica conjugated with
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
The ability of total homogenate (TH) of Fasciola hepatica conjugated with
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
The ability of total homogenate (TH) of Fasciola hepatica conjugated with
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
Fasciola hepatica conjugated with
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
The ability of total homogenate (TH) of Fasciola hepatica conjugated with
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
The ability of total homogenate (TH) of Fasciola hepatica conjugated with
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
The ability of total homogenate (TH) of Fasciola hepatica conjugated with
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.
aluminium hydroxide (alum) or Freunds complete adjuvant (FCA) to protect
cattle against experimental fasciolosis was evaluated. Compared with the
infected group, the immunized animals with alum-TH and FCA-TH presented a
significant reduction in fluke burden (85.9% and 96.8%, respectively), a higher
percentage of short-sized worms, a marked reduction in the released eggs in
faeces (89% and 57%, respectively), as well as an increased production of specific
antibodies before infection. The alum-TH immunized group also showed a
significant increase in the antigen-specific proliferation of peripheral blood
mononuclear cells (PBMC) as early as 4 weeks before infection. Although both
immunized groups (alum-TH and FCA-TH) were able to develop an efficient
protective immune response to metacercarial challenge, an earlier PBMC
response, lower hepatic damage and less effect on weight gain were found in
alum-immunized animals. Therefore, alum is a good candidate for future
immunization against bovine fasciolosis.