Adoptive transfer of dendritic cells pulsed with Fasciola hepatica antigens and LPS confers

FALCON, C; CARRANZA, F; AOKI, MP; SANCHEZ, MC; MOTRAN, CLAUDIA C; CERVI, LAP

JOURNAL OF INFECTIOUS DISEASES

UNIV CHICAGO PRESS

Año: 2012 vol. 205 p. 506 - 514

0022-1899

Abstract: Dendritic cells (DC) can function as adjuvants able to mediate protection against different pathogens. Given that successful vaccination against F. hepatica is mostly related to the induction of Th1 responses, we studied the potential of DC loaded with F. hepatica Ags and LPS (which promote DC maturation) as a vaccine against fasciolosis in BALB/c mice. However, only a semi-mature phenotype was achieved when DC were simultaneously cultured with a F. hepatica total extract (TE) and LPS. The activation status of TE loaded DC was enhanced when these cells were treated with TE 90 min before being stimulated with LPS (TE90 DC). More importantly, a single immunization of mice with TE90 DC stimulated a systemic Th1 response and conferred protection against hepatic damage induced by F. hepatica infection. Thus, TE90 DC may prove to be a useful new tool for vaccination against F. hepatica.