CIBICI 14215
CENTRO DE INVESTIGACION EN BIOQUIMICA CLINICA E INMUNOLOGIA
Unidad Ejecutora - UE
artículos
Título:
Safety levels of systemic IL-12 induced by cDNA expression as a cancer therapeutic
Autor/es:
BAEZ, NATALIA S.; YOUNG, HOWARD A.; VIANO, MARIA ESTEFANIA; MARIANA MATELLON; CONSTANZA SAVID-FRONTERA; DELLA REYNOLDS; MARIA CECILIA RODRIGUEZ GALAN
Revista:
IMMUNOTHERAPY
Editorial:
FUTURE MEDICINE LTD
Referencias:
Lugar: Londres; Año: 2021
ISSN:
1750-743X
Resumen:
Aims: The aim of this work is to utilize a gene expression procedure to safety express systemic IL-12 and evaluate its effects in mouse tumor models. Materials & Methods: SLO and tumors from EL4 and B16 tumor bearing mice were analyzed by supervised and unsupervised methods. Results: IL-12 cDNA induced systemic IL-12 protein levels lower than the tolerated dose in patients. Control of tumor growth is observed in s.c. B16 and EL-4 tumors. Systemic IL-12 expression induced a higher frequency of both total tumor-infiltrated CD45+ cells and proliferative IFN+CD8+T cells along with a lower frequency of CD4+Foxp3+ and CD11b+Gr-1+ cells. Conclusions: This approach characterizes the systemic effects of IL-12 helping to improved treatment of metastases or solid tumors.
