Regulation of Lipid Biosynthesis in Gram-Positive Bacteria: Role of the Asp23 Family Protein YqhY and the End Product of the Pathway Acil-ACP

ALBANESI, DANIELA; DE MENDOZA, DIEGO; MACHINANDIARENA, FEDERICO

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias. LIII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2017

Sociedades de Biociencias - Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Bacteria strictly control the synthesis of their membrane phospholipids in response to different nutritional conditions, but the underlying regulatory mechanisms are not fully known yet. The acetyl-CoA carboxylase (ACC) produces malonyl-CoA, a metabolite that is not only a substrate of FASII but also a key regulator of this biosynthetic pathway as it controls the activity of the global repressor FapR and hence the expression of the fap regulon in Firmicutes. In this work, we focused on YqhY, which belongs to the family of proteins without known function Asp23. It has been recently reported that a Bacillus subtilis ΔyqhY mutant rapidly acquire suppressor mutations that affect any of the subunits of the ACC, suggesting that YqhY could be capable of modulating lipid synthesis. In this work, we analyzed [14C]-acetate incorporation into the lipid fraction of a B. subtilis ΔyqhY mutant and observed a 220% increase with respect to the isogenic wild type strain (p