IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Cell type specific compartmentalization of the Bacillus subtilis stress response transcription factor Sigma B
Autor/es:
ANIBAL GOÑI; MARCELO B. MENDEZ; ROBERTO GRAU
Lugar:
Mar del Plata
Reunión:
Congreso; XLIII Reunión Nacional de la Sociedad Argentina de investigación en Bioquímica y Biología Molecular; 2007
Institución organizadora:
SAIB
Resumen:
B. subtilis is a soil bacterium, and hence temperature changes,
starvation, and solar radiation would constitute common
environmental stresses. Stress resistance in is governed
by the master transcription factor sigma B (SigB). We showed in
previous reports that SigB is dramatically induced after a
temperature downshift from 37°C to 20°C. Loss of SigB reduces
stationary-phase viability of cold-adapted cells 10 to 15-fold. Here,
we show that SigB is exclusively trapped in the pre-spore
compartment of during the development of the spore. The
presence in the spore prepares it for future stress. Spores of sigB
mutants present a significant reduction in germination rate
compared to the wild type strain in alcohol stressing germination
medium. Sigma B is shown to be necessary for appropriated
function of germination receptors (GerBK) under stress conditions.
Moreover, it was observed a delay in the outgrowth for the sigB
mutant when we compared it with wild type spores under the same
conditions. Importantly, we found a decrease in the viability of
mutant spores when they were exposed to extreme conditions as acid
or UV-C light. These experiments are discussed in the context of the
lithopanspermia theory and the novel role, discovered in this work,
of sigmaBas a Trojan horse hidden in the dormant spore.
starvation, and solar radiation would constitute common
environmental stresses. Stress resistance in is governed
by the master transcription factor sigma B (SigB). We showed in
previous reports that SigB is dramatically induced after a
temperature downshift from 37°C to 20°C. Loss of SigB reduces
stationary-phase viability of cold-adapted cells 10 to 15-fold. Here,
we show that SigB is exclusively trapped in the pre-spore
compartment of during the development of the spore. The
presence in the spore prepares it for future stress. Spores of sigB
mutants present a significant reduction in germination rate
compared to the wild type strain in alcohol stressing germination
medium. Sigma B is shown to be necessary for appropriated
function of germination receptors (GerBK) under stress conditions.
Moreover, it was observed a delay in the outgrowth for the sigB
mutant when we compared it with wild type spores under the same
conditions. Importantly, we found a decrease in the viability of
mutant spores when they were exposed to extreme conditions as acid
or UV-C light. These experiments are discussed in the context of the
lithopanspermia theory and the novel role, discovered in this work,
of sigmaBas a Trojan horse hidden in the dormant spore.
starvation, and solar radiation would constitute common
environmental stresses. Stress resistance in is governed
by the master transcription factor sigma B (SigB). We showed in
previous reports that SigB is dramatically induced after a
temperature downshift from 37°C to 20°C. Loss of SigB reduces
stationary-phase viability of cold-adapted cells 10 to 15-fold. Here,
we show that SigB is exclusively trapped in the pre-spore
compartment of during the development of the spore. The
presence in the spore prepares it for future stress. Spores of sigB
mutants present a significant reduction in germination rate
compared to the wild type strain in alcohol stressing germination
medium. Sigma B is shown to be necessary for appropriated
function of germination receptors (GerBK) under stress conditions.
Moreover, it was observed a delay in the outgrowth for the sigB
mutant when we compared it with wild type spores under the same
conditions. Importantly, we found a decrease in the viability of
mutant spores when they were exposed to extreme conditions as acid
or UV-C light. These experiments are discussed in the context of the
lithopanspermia theory and the novel role, discovered in this work,
of sigmaBas a Trojan horse hidden in the dormant spore.
starvation, and solar radiation would constitute common
environmental stresses. Stress resistance in is governed
by the master transcription factor sigma B (SigB). We showed in
previous reports that SigB is dramatically induced after a
temperature downshift from 37°C to 20°C. Loss of SigB reduces
stationary-phase viability of cold-adapted cells 10 to 15-fold. Here,
we show that SigB is exclusively trapped in the pre-spore
compartment of during the development of the spore. The
presence in the spore prepares it for future stress. Spores of sigB
mutants present a significant reduction in germination rate
compared to the wild type strain in alcohol stressing germination
medium. Sigma B is shown to be necessary for appropriated
function of germination receptors (GerBK) under stress conditions.
Moreover, it was observed a delay in the outgrowth for the sigB
mutant when we compared it with wild type spores under the same
conditions. Importantly, we found a decrease in the viability of
mutant spores when they were exposed to extreme conditions as acid
or UV-C light. These experiments are discussed in the context of the
lithopanspermia theory and the novel role, discovered in this work,
of sigmaBas a Trojan horse hidden in the dormant spore.
is a soil bacterium, and hence temperature changes,
starvation, and solar radiation would constitute common
environmental stresses. Stress resistance in is governed
by the master transcription factor sigma B (SigB). We showed in
previous reports that SigB is dramatically induced after a
temperature downshift from 37°C to 20°C. Loss of SigB reduces
stationary-phase viability of cold-adapted cells 10 to 15-fold. Here,
we show that SigB is exclusively trapped in the pre-spore
compartment of during the development of the spore. The
presence in the spore prepares it for future stress. Spores of sigB
mutants present a significant reduction in germination rate
compared to the wild type strain in alcohol stressing germination
medium. Sigma B is shown to be necessary for appropriated
function of germination receptors (GerBK) under stress conditions.
Moreover, it was observed a delay in the outgrowth for the sigB
mutant when we compared it with wild type spores under the same
conditions. Importantly, we found a decrease in the viability of
mutant spores when they were exposed to extreme conditions as acid
or UV-C light. These experiments are discussed in the context of the
lithopanspermia theory and the novel role, discovered in this work,
of sigmaBas a Trojan horse hidden in the dormant spore.