Transcriptome Response to Potato virus X 25K effector in Solanum tuberosum

MARTINEZ, MA. FLORENCIA; GARCIA, LUCILA; MARANO, MA. ROSA; MARTIN, ANA PAULA; GADEA, J.; TASSELLI, SABRINA E.; CHOROSTECKI, UCIEL

Online

Congreso; XXXIII Argentinian meeting of Plant Physiology; 2021

Sociedad Argentina de Fisiología Vegetal

Potato (Solanum tuberosum L.) is the fourth most important crop worldwide. Nb is a single dominant gene from cv. Pentland Ivory that confers hypersensitive resistance (HR) to potato virus X (PVX) strain ROTH1, which carries the 25-kDa effector protein (25K1). The gene 25K of the virulent strain UK3 (25K3) overcomes the HR. However, the molecular mechanisms governing the incompatible (Nb-25K1) and compatible (Nb-25K3) interaction are unknown. In this study, an integrated microRNA and mRNA transcriptome analysis of Nb potato leaves infiltrated with Agrobacterium cultures harbouring pBIN25K1, pBIN25K3 or empty vector was performed. Eleven miRNAs (eight known and three novel), and three miRNAs (one known and two novel) were identified in response to 25K1 and 25K3, respectively. We performed target predictions for differentially expressed miRNAs and correlated their expression levels through mRNA sequencing data. GO analysis of the targets revealed an overrepresentation of genes involved in cell wall reinforcement, oxidative burst, accumulation of phenolic compounds and cell death in the incompatible interaction. The biological processes associated with strengthening of cell wall that involves the miRNAs -stu-miR397 and stu-miR408- which target laccases, were validated by qRT-PCR and histochemical analysis. This study provides a deeper understanding of miRNA dependent regulatory networks in the resistance response to PVX-25K.