Analysis of Expression Gradients of Developmental Regulators in Arabidopsis thaliana Roots

GOLDY, CAMILA; VENA, RODRIGO; RODRIGUEZ, RAMIRO E.; ERCOLI, MARIA FLORENCIA; PALATNIK, JAVIER F.

Morphogen Gradients. Methods in Molecular Biology.

Humana Press, New York, NY

Lugar: New York; Año: 2018; p. 3 - 17

The regulatory mechanisms involved in plant development include many signals, some of them acting as graded positional cues regulating gene expression in a concentration-dependent manner. These regulatory molecules, that can be considered similar to animal morphogens, control cell behavior in developing organs. A suitable experimental approach to study expression gradients in plants is quantitative laser scanning confocal microscopy (LSCM) using Arabidopsis thaliana root tips as a model system. In this chapter, we outline a detailed method for image acquisition using LSCM, including detailed microscope settings and image analysis using FIJI as software platform.