IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
capítulos de libros
Título:
Rhodobacter capsulatus FerredoxinVI and Ferredoxin(Flavodoxin)-NADP(H) Oxidoreductase are redox partners in vitro
Autor/es:
VERÓNICA DUMIT; ANA BORTOLOTTI; NÉSTOR CARRILLO; NÉSTOR CORTEZ; YVES JOUANNEAU; MILAGROS MEDINA
Libro:
Flavins and Flavoproteins 2008
Editorial:
Prensas Universitarias de Zaragoza
Referencias:
Lugar: Zaragoza; Año: 2008; p. 107 - 112
Resumen:
Ferredoxin(flavodoxin)-NADP(H) oxidoreductase (FPR) from the photosynthetic bacterium Rhodobacter capsulatus is a FAD-containing enzyme that reduces one-electron carrier proteins, such as ferredoxins (Fd) and flavodoxins (Fld), by means of NADPH. FPR is able to transfer electrons to nitrogenase via the reduction of the flavodoxin NifF, its single protein substrate assayed so far [1]. We described here the interaction between FPR and FdVI. Even though this electron carrier has no assigned function, it has been recently related to iron-sulfer clusters biosynthesis in Rhodobacter capsulatus [2]. The analysis of their interaction was performed through in vitro and in silico studies: stopped-flow, differential absorption, steady-state kinetics and a docking assay was carried out from their crystallographic structures [2,3] in order to characterize the putative complex they attain, not yet resolved by X-ray crystallography for any bacterial system. The results presented herein suggest that the FPR-FdVI interaction can not be fit to a single stable complex formation, and would be better described by a number of transient contacts between the proteins. Different orientations could all contribute to the electron transfer reactions, as it has been proposed for Anabaena complexes [4].         [1] Bittel C, Tabares LC, Armesto M, Carrillo N, Cortez N (2003) FEBS Lett. 553,408-412. [2] Sainz, G.; Jakoncic, J.; Sieker, L.C.; Stojanoff, V.; Sanishvili, N.; Asso, M.; Bertrand, P.; Armengaud, J. And Jouanneau, Y. (2006) J Biol Inorg Chem. 11, 235-46 [3] Nogués I, Pérez-Dorado I, Frago S, Bittel C, Mayhew SG, Gómez-Moreno C. Hermoso JA, Medina M, Cortez N, Carrillo N (2005) Biochemistry 4, 11730-11740. [4] Medina, M.; Abagyan, R.; Gómez-Moreno, C.; Fernandez-Reccio, J. (2008) Proteins (in press).