IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Zoonotic transmission of hepatitis E virus in a pig farmer from Argentina: A case report
Autor/es:
JULIÁN ACOSTA; ALEJANDRO COSTAGUTA; DANIELA GARDIOL; FEDERICO MARZIALI; HUGO TANNO; ANA LAURA CAVATORTA; ALCEO GALIMBERTI; FERNANDO BESSONE; MARÍA VIRGINIA REGGIARDO
Revista:
ZOONOSES AND PUBLIC HEALTH
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2021
ISSN:
1863-1959
Resumen:
Hepatitis E virus (HEV) is a public health concern due to its zoonotic transmission tohuman, being pigs a highly recognized reservoir. We previously demonstrated HEVgenotype 3 infections in pig herds from the highest commercial active region fromArgentina. Here, we present a case of acute symptomatic hepatitis E in an elderly manwith occupational exposure to pigs who referred regular consumption of pork andsausages. HEV infection in this patient was demonstrated by serological methods, aswell as by HEV RNA detection in serum and stool samples using the HEV/MS2 duplexRT-qPCR, formerly optimized in our laboratory. We further detected HEV RNA in pigfaeces from the patient´s farm. To confirm the potential role of swine in the transmission, we performed a phylogenetic analysis of all HEV RNA derived from both, thepatient and the pig samples. A 303 nt region within the HEV 5 ´ORF2 was amplified bynested RT-PCR and subsequently sequenced. Phylogenetic analysis showed that thestrains isolated from the farmer and from his pigs presented a nucleotide identity of100%. These results support the zoonotic transmission of circulating HEV strains andconfirm this epidemiological association for the first time in Argentina.