IBR 13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Hormone-regulated PKA activity in porcine oviductal epithelial cells
Autor/es:
MARINI, PATRICIA ESTELA; TEIJEIRO, JUAN MANUEL
Revista:
CELL AND TISSUE RESEARCH
Editorial:
SPRINGER
Referencias:
Año: 2020 vol. 380 p. 657 - 667
ISSN:
0302-766X
Resumen:
The oviduct is a dynamic organ that suffers changes during the oestrous cycle and modulates gamete and embryo physiology.Weanalyse the possible existence of Protein kinase A (PKA)-dependent hormone-regulated pathways in porcine ampulla andprimary cell cultures by 2D-electrophoresis/Western blot using anti-phospho PKA substrate antibodies. Differential phosphorylationwas observed for ten proteins that were identified by mass spectrometry. The results were validated for five of theproteins: Annexin A5, Calumenin, Glyoxalase I and II and Enolase I. Immunofluorescence analyses show that Calumenin,Glyoxalase II and Enolase I change their localisation in the oviductal epithelium through the oestrus cycle. The resultsdemonstrate the existence of PKA hormone-regulated pathways in the ampulla epithelium during the oestrus cycle.
