IBR 13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Parallel screening and optimization of protein constructs for structural studies
Autor/es:
RASIA RM; NOIRCLERC-SAVOYE M; BOLOGNA NG; GALLET B; PLEVIN MJ; BLANCHARD L; PALATNIK JF; BRUTSCHER B; VERNET T; BOISBOUVIER J
Revista:
PROTEIN SCIENCE
Referencias:
Año: 2009 vol. 18 p. 434 - 439
ISSN:
0961-8368
Resumen:
A major challenge in structural biology remains the identification of protein constructs amenable to structural characterization. Here, we present a simple method for parallel expression, labeling, and purification of protein constructs (up to 80 kDa) combined with rapid evaluation by NMR spectroscopy. Our approach, which is equally applicable for manual or automated implementation, offers an efficient way to identify and optimize protein constructs for NMR or X-ray crystallographic investigations.
