IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Cloning, characterization and subcellular localization of a trypanosoma cruzi argonaute protein defining a new subfamily distinctive of trypanosomatids
Autor/es:
GARCIA SILVA, MR; TOSAR, TJ; FRUGIER, M; PANTANO, S; BONILLA, B; ESTEBAN, L; SERRA ESTEBAN; ROBIRA, C; ROBELLO, C; CAYOTA, A
Revista:
GENE
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Año: 2010
ISSN:
0378-1119
Resumen:
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Over the last years an expanding family of small
non-coding RNAs (sRNA) has been identified in eukaryoticgenomes which behave as
sequence-specific triggers for mRNA degradation,
translation repression, heterochromatin formation and genome stability. To
achieve their effectors functions, sRNAs associate with members of the
Argonaute protein family. Argonaute proteins are segregated into three
paralogous groups: the AGO-like subfamily, the PIWI-like subfamily, and the
WAGO subfamily (for Worm specific AGO). Detailed phylogenetic analysis
of the small RNA-related machinery components revealed that they can be traced
back to the common ancestor of eukaryotes. However, this machinery seems to be
lost or excessively simplified in some unicellular organisms such as
Saccharomyces
cerevisiae, Trypanosoma cruzi, Leishmania major and Plasmodium
falciparum which are unable to
utilize dsRNA to trigger degradation of target RNAs. We reported here a unique
ORF encoding for an AGO/PIWI protein in T. cruzi which was expressed in all stages of its life cycle at the transcript as
well as the protein level. Database search for remote homologues, revealed the presence
of a divergent PAZ domain adjacent to the well supported PIWI domain. Our
results strongly suggested that this unique AGO/PIWI protein from T. cruzi is a canonical Argonaute in terms of its domain architecture.
We propose to reclassify all Argonaute members from trypanosomatids as a
distinctive phylogenetic group representing a new subfamily of Argonaute
proteins and propose the generic designation of AGO/PIWI-tryp to identify them.
Inside the Trypanosomatid-specific node, AGO/PIWI-tryps were clearly segregated
into two paralog groups designated as AGO-tryp and PIWI-tryp according to the
presence or absence of a functional link with RNAi-related phenomena,
respectively.