Sperm binding to porcine oviductal cells is mediated by SRCR domains contained in DMBT1

TEIJEIRO, JUAN MANUEL; ROLDAN MARIA LORENA; MARINI, PATRICIA ESTELA; ROLDAN MARIA LORENA; MARINI, PATRICIA ESTELA; RUIZ ÁLVAREZ, JIMENA; RUIZ ÁLVAREZ, JIMENA; TEIJEIRO, JUAN MANUEL

JOURNAL OF CELLULAR BIOCHEMISTRY

WILEY-LISS, DIV JOHN WILEY & SONS INC

Año: 2018 vol. 119 p. 3755 - 3762

0730-2312

The oviduct is an organ in which a subpopulation of sperm is stored in a reservoir, preserving its fertilizing potential. In porcine, two oviductal proteins have been identified in relation to sperm binding, Annexin A2 and Deleted in Malignant Brain Tumor 1 (DMBT1). DMBT1 is a multifunctional, multidomain glycoprotein, and the characteristics of all of its domains, as well as its carbohydrates, make them candidates for sperm binding. In this work, we challenge sperm for binding to pig oviductal cells on primary culture, after treatment with antibodies specific for the different domains present in DMBT1. Only anti-SRCR antibodies produced inhibition of sperm binding to cells. Thus, SRCR is the main domain in DMBT1 promoted sperm binding to form the reservoir in the oviduct, and this function is probably elicited through the polypeptide itself.