IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Glutamine protection in an experimental model of acetaminophen nephrotoxicity
Autor/es:
MOLINAS SARA MARÍA,; BROVEDAN, M. A.,; LAURA TRUMPER; LILIANA A. MONASTEROLO,; GERARDO PISANI,
Revista:
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
Editorial:
NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS
Referencias:
Lugar: Otawa; Año: 2017 vol. 96 p. 366 - 371
ISSN:
0008-4212
Resumen:
Acetaminophen (APAP) is a widely prescribed analgesic - antipyretic drug. In thepresent work we studied the effects of glutamine (Gln) in an ?in vivo? model of APAPinduced nephrotoxicity in male Wistar rats. Renal function, histological characteristics and Na+, K+ ATPase cortical abundance and distribution were analyzed. The appearance of HSP70 and actin in urine were also evaluated. Mieloperoxidase activity in cortical tissue was measured as an index of the inflammatory response. Gln administration 30 min before APAP protected from the renal functional and histological damage promoted by APAP. Rats that received the dual treatment Gln and APAP (Gln/APAP) showed the same level of Na+, K+ ATPase cortical induction than APAP treated animals, but the enzyme maintained its normal basolateral localization. HSP70 abundance was increased up to the same level in Gln, APAP, or Gln/APAP groups. Urinary HSP70 and actin were detected only in the APAP treated animals, reinforcing the protection of renal tubular integrity afforded by the Gln pretreatment. Gln pretreatment also protected from the increment in MPO activity promoted by APAP.Our results support the idea that Gln pretreatment could be a therapeutic option to prevent APAP-induced renal injury.