IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Identification of FadAB Complexes Involved in Fatty Acid b-Oxidation in Streptomyces coelicolor and Construction of a Triacylglycerol Overproducing strain
Autor/es:
SIMÓN MENENDEZ-BRAVO, JULIÁN PAGANINI, CLAUDIO AVIGNONE-ROSSA, HUGO GRAMAJO, ANA ARABOLAZA; SIMÓN MENENDEZ-BRAVO, JULIÁN PAGANINI, CLAUDIO AVIGNONE-ROSSA, HUGO GRAMAJO, ANA ARABOLAZA
Revista:
Frontiers in microbiology
Editorial:
Frontiers
Referencias:
Año: 2017
Resumen:
Oleaginous microorganisms represent possible platforms for the sustainable productionof oleochemicals and biofuels due to their metabolic robustness and the possibilityto be engineered. Streptomyces coelicolor is among the narrow group of prokaryotescapable of accumulating triacylglycerol (TAG) as carbon and energy reserve. Although thepathways for TAG biosynthesis in this organism have been widely addressed, the set ofgenes required for their breakdown have remained elusive so far. Here, we identified andcharacterized three gene clusters involved in the b-oxidation of fatty acids (FA). The role ofeach of the three different S. coelicolor FadAB proteins in FA catabolism was confirmedby complementation of an Escherichia coli1fadBA mutant strain deficient in b-oxidation.In S. coelicolor, the expression profile of the three gene clusters showed variation relatedwith the stage of growth and the presence of FA in media. Flux balance analyses usinga corrected version of the current S. coelicolor metabolic model containing detailedTAG biosynthesis reactions suggested the relevance of the identified fadAB genes inthe accumulation of TAG. Thus, through the construction and analysis of fadAB knockoutmutant strains, we obtained an S. coelicolor mutant that showed a 4.3-fold increase in theTAG content compared to the wild type strain grown under the same culture conditions.